Wrapping Nanocrystals with an Amphiphilic Polymer Preloaded with Fixed Amounts of Fluorophore Generates FRET-Based Nanoprobes with a Controlled Donor/Acceptor Ratio

Printer-friendly version
TitreWrapping Nanocrystals with an Amphiphilic Polymer Preloaded with Fixed Amounts of Fluorophore Generates FRET-Based Nanoprobes with a Controlled Donor/Acceptor Ratio
Type de publicationJournal Article
Nouvelles publications2009
AuteursYakovlev AV, Zhang F, Zulqurnain A, Azhar-Zahoor A, Luccardini C, Gaillard S, Mallet J-M, Tauc P, Brochon JC, Parak WJ, Feltz A, Oheim M
JournalLangmuir
Volume25
Fascicule5
Pagination3232-3239
Année de publicationMar
Numéro0743-7463
Accession NumberISI:000263770800104
Résumé

Colloidal nanocrystal (NC) donors wrapped with a polymer coating including multiple organic acceptor molecules are promising scaffolds for fluorescence resonance energy transfer (FRET)-based nanobiosensors. Over other self-assembling donor-acceptor configurations, our preloaded polymers have the virtue of producing compact assemblies with a fixed donor/acceptor distance. This property, together with the possibility of stoichiometric polymer loading, allowed us to directly address how the FRET efficiency depended on the donor/acceptor. At the population level, nanoprobes based on commercial as well as custom CdSe/ZnS donors displayed the expected dose-dependent rise in transfer efficiency, saturating from about five ATTO dyes/NC. However, for a given acceptor concentration, both the intensity and lifetime of single-pair FRET data revealed a large dispersion of transfer efficiencies, highlighting an important heterogeneity among nominally identical FRET-based nanoprobes. Rigorous quality check during synthesis and shell assembly as well as postsynthesis sorting and purification are required to make hybrid semiconductor-organic nanoprobes a robust and viable alternative to organic or genetically encoded nanobiosensors.

URL<Go to ISI>://000263770800104
DOI10.1021/la8038347
Importer un fichierWrapping Nanocrystals with an Amphiphilic Polymer Preloaded with Fixed Amounts of Fluorophore Generates FRET-Based Nanoprobes with a Controlled Donor/Acceptor Ratio
Unité de rattachement: 
Departement