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Proteomic comparison of the EWS-FLI1 expressing cells EF with NIH-3T3 and actin remodeling effect of (R/W)9 cell-penetrating peptide


EuPA Open Proteomics 2016;10:1-8


EWS-FLI1 expression in NIH-3T3 fibroblasts has a profound impact on the phenotype, resulting in the cytoskeleton and adhesive capacity disorganization (EF cells). Besides this, (R/W)9, a cell-penetrating peptide (CPP), has an intrinsic actin remodeling activity in EF cells. To evaluate the impact of the oncogenic protein EWS-FLI1 on proteins expression levels, a quantitative comparison of tumoral EF and non-tumoral 3T3 proteomes was performed. Then to see if we could link the EWS-FLI1 oncogenic transformation to the phenotype reversion induced by (R/W)9, (R/W)9 influence on EF cells proteome was assessed. To our knowledge no such “CPPomic” study has been performed before.



Entasis through Hook-and-Loop Fastening in a Glycoligand with Cumulative Weak Forces Stabilizing Cu(I)

J. Am. Chem. Soc.2015

The idea of a possible control of metal ion properties by constraining the coordination sphere geometry was introduced by Vallee and Williams with the concept of entasis, which is frequently postulated to be at stake in metallobiomolecules. However, the interactions controlling the geometry at metal centers remain often elusive. In this study, the coordination properties toward copper ions - Cu(II) or Cu(I) - of a geometrically constrained glycoligand centered on a sugar scaff old were compared with those of an analogous ligand built on an unconstrained alkyl chain. The sugar-centered ligand was shown to be more preorganized for Cu(II)  coordination than its open-chain analogue, with an unusual additional stabilization of the Cu(I)  redox state.

An easy-to-detect nona-arginine peptide for epidermal targeting


Chem. Commun., 2015, 51, 2687-2689


A correlative approach combining synchrotron radiation based IR microscopy and fluorescence microscopy enabled the successful detection and quantification of a nona-arginine peptide labelled with a Single Core Multimodal Probe for Imaging (SCoMPI) in skin biopsies. The topical penetration of the conjugate appeared to be time dependent and occurred most probably via the extracellular matrix.



Nanosecond motions in proteins revealed by high-resolution relaxometry

A proper description of biological processes at the atomic level require a full characterization of both the structure and the dynamics of biomolecules. Nuclear magnetic resonance (NMR) is a method of choice to access both to the structure and the dynamics of proteins and nucleic acids. One of most powerful NMR probes of biomolecular dynamics is nuclear spin relaxation. Here, we show that nanosecond time scale motions can be revealed with an emerging technique: high-resolution relaxometry.

Membrane tubulation by cell penetrating peptides

Cell penetrating peptides induce membrane invaginations in cellular membranes. They induce negative membrane curvature by a metabolic energy independent pathway. This pathway also called 'Physical endocytosis' could be a new mechanism of cell penetrating peptide uptake